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Effectiveness of esterified whey proteins fractions against Egyptian Lethal Avian Influenza A (H5N1).

ABSTRACT: BACKGROUND:Avian influenza A (H5N1) virus is one of the most important public health concerns worldwide. The antiviral activity of native and esterified whey proteins fractions (?- lactalbumin, ?- lactoglobulin, and lactoferrin) was evaluated against A/chicken/Egypt/086Q-NLQP/2008 HPAI (H5N1) strain of clade 2.2.1 (for multiplicity of infection (1 MOI) after 72 h of incubation at 37 °C in the presence of 5% CO?) using MDCK cell lines. RESULT:Both the native and esterified lactoferrin seem to be the most active antiviral protein among the tested samples, followed by ?- lactoglobulin. ?-Lactalbumin had less antiviral activity even after esterification. CONCLUSION:Esterification of whey proteins fractions especially lactoferrin and ?-lactoglobulin enhanced their antiviral activity against H5N1 in a concentration dependent manner.

SUBMITTER: Taha SH

PROVIDER: S-EPMC2998487 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Effectiveness of esterified whey proteins fractions against Egyptian Lethal Avian Influenza A (H5N1).

Taha Soad H SH Mehrez Mona A MA Sitohy Mahmoud Z MZ Abou Dawood Abdel Gawad I AG Abd-El Hamid Mahmoud M MM Kilany Walid H WH

Virology journal 20101119

<h4>Background</h4>Avian influenza A (H5N1) virus is one of the most important public health concerns worldwide. The antiviral activity of native and esterified whey proteins fractions (α- lactalbumin, β- lactoglobulin, and lactoferrin) was evaluated against A/chicken/Egypt/086Q-NLQP/2008 HPAI (H5N1) strain of clade 2.2.1 (for multiplicity of infection (1 MOI) after 72 h of incubation at 37 °C in the presence of 5% CO₂) using MDCK cell lines.<h4>Result</h4>Both the native and esterified lactofer ...[more]

PMID: 21092081

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Project description:Although vaccination of poultry for control of highly pathogenic avian influenza virus (HPAIV) H5N1 has been practiced during the last decade in several countries, its effectiveness under field conditions remains largely unquantified. Effective HPAI vaccination is however essential in preventing incursions, silent infections and generation of new H5N1 antigenic variants. The objective of this study was to asses the level and duration of vaccine induced immunity in commercial layers in Indonesia. Titres of H5N1 haemagglutination inhibition (HI) antibodies were followed in individual birds from sixteen flocks, age 18-68 week old (wo). The study revealed that H5N1 vaccination had highly variable outcome, including vaccination failures, and was largely ineffective in providing long lasting protective immunity. Flocks were vaccinated with seven different vaccines, administer at various times that could be grouped into three regimes: In regime A, flocks (n = 8) were vaccinated two or three times before 19 wo; in regime B (n = 2), two times before and once after 19 wo; and in regime C (n = 6) three to four times before and two to three times after 19 wo. HI titres in regime C birds were significantly higher during the entire observation period in comparison to titres of regime A or B birds, which also differed significantly from each other. The HI titres of individual birds in each flock differed significantly from birds in other flocks, indicating that the effectiveness of field vaccination was highly variable and farm related. Protective HI titres of >4log2, were present in the majority of flocks at 18 wo, declined thereafter at variable rate and only two regime C flocks had protective HI titres at 68 wo. Laboratory challenge with HPAIV H5N1 of birds from regime A and C flocks confirmed that protective immunity differed significantly between flocks vaccinated by these two regimes. The study revealed that effectiveness of the currently applied H5N1 vaccination could be improved and measures to achieve this are discussed.

Dataset Information

Effectiveness of esterified whey proteins fractions against Egyptian Lethal Avian Influenza A (H5N1).

Publications

Effectiveness of esterified whey proteins fractions against Egyptian Lethal Avian Influenza A (H5N1).

Similar Datasets

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