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Coding potential of laboratory and clinical strains of human cytomegalovirus.

ABSTRACT: Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

SUBMITTER: Murphy E

PROVIDER: S-EPMC299866 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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Coding potential of laboratory and clinical strains of human cytomegalovirus.

Murphy Eain E Yu Dong D Grimwood Jane J Schmutz Jeremy J Dickson Mark M Jarvis Michael A MA Hahn Gabriele G Nelson Jay A JA Myers Richard M RM Shenk Thomas E TE

Proceedings of the National Academy of Sciences of the United States of America 20031201 25

Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isola ...[more]

PMID: 14657367

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