Ontology highlight
ABSTRACT:
SUBMITTER: Lee G
PROVIDER: S-EPMC2999004 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Lee Gwangrog G Hartung Sophia S Hopfner Karl-Peter KP Ha Taekjip T
Nano letters 20101117 12
Molecular motors have inspired many avenues of research for nanotechnology but most molecular motors studied so far allow only unidirectional movement. The archaeal RNA-exosome is a reversible motor that can either polymerize or degrade an RNA strand, depending on the chemical environments. We developed a single molecule fluorescence assay to analyze the real time locomotion of this nanomachine on RNA. Despite the multimeric structure, the enzyme followed the Michaelis-Menten kinetics with the m ...[more]