Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation.
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ABSTRACT:
SUBMITTER: Jo H
PROVIDER: S-EPMC2999665 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
Selective incorporation of nitrile-based infrared probes into proteins via cysteine alkylation.
Jo Hyunil H Culik Robert M RM Korendovych Ivan V IV Degrado William F WF Gai Feng F
Biochemistry 20101117 49
The nitrile stretching vibration is increasingly used as a sensitive infrared probe of local protein environments. However, site-specific incorporation of a nitrile moiety into proteins is difficult. Here we show that various aromatic nitriles can be easily incorporated into peptides and proteins via either thiol alkylation or arylation reaction. ...[more]
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