Functional and pathogenic differences of Th1 and Th17 cells in experimental autoimmune encephalomyelitis.

ABSTRACT:

Background

There is consensus that experimental autoimmune encephalomyelitis (EAE) can be mediated by myelin specific T cells of Th1 as well as of Th17 phenotype, but the contribution of either subset to the pathogenic process has remained controversial. In this report, we compare functional differences and pathogenic potential of "monoclonal" T cell lines that recognize myelin oligodendrocyte glycoprotein (MOG) with the same transgenic TCR but are distinguished by an IFN-γ producing Th1-like and IL-17 producing Th17-like cytokine signature.

Methods and findings

CD4+ T cell lines were derived from the transgenic mouse strain 2D2, which expresses a TCR recognizing MOG peptide 35-55 in the context of I-A(b). Adoptive transfer of Th1 cells into lymphopenic (Rag2⁻/⁻) recipients,

SUBMITTER: Domingues HS 

PROVIDER: S-EPMC3000428 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature