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The homodimeric GBS1074 from Streptococcus agalactiae.

ABSTRACT: ESAT-6 is a well characterized secreted protein from Mycobacterium tuberculosis and represents the archetype of the WXG100 family of proteins. Genes encoding ESAT-6 homologues have been identified in the genome of the human pathogen Streptococcus agalactiae; one of these genes, esxA, has been cloned and the recombinant protein has been crystallized. In contrast to M. tuberculosis ESAT-6, the crystal structure of GBS1074 reveals a homodimeric structure similar to homologous structures from Staphylococcus aureus and Helicobacter pylori. Intriguingly, GBS1074 forms elongated fibre-like assemblies in the crystal structure.

SUBMITTER: Shukla A

PROVIDER: S-EPMC3001639 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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The homodimeric GBS1074 from Streptococcus agalactiae.

Shukla Anshuman A Pallen Mark M Anthony Mark M White Scott A SA

Acta crystallographica. Section F, Structural biology and crystallization communications 20101027 Pt 11

ESAT-6 is a well characterized secreted protein from Mycobacterium tuberculosis and represents the archetype of the WXG100 family of proteins. Genes encoding ESAT-6 homologues have been identified in the genome of the human pathogen Streptococcus agalactiae; one of these genes, esxA, has been cloned and the recombinant protein has been crystallized. In contrast to M. tuberculosis ESAT-6, the crystal structure of GBS1074 reveals a homodimeric structure similar to homologous structures from Staphy ...[more]

PMID: 21045286

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