SNP discovery in the bovine milk transcriptome using RNA-Seq technology.
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ABSTRACT:
SUBMITTER: Canovas A
PROVIDER: S-EPMC3002166 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
SNP discovery in the bovine milk transcriptome using RNA-Seq technology.
Cánovas Angela A Rincon Gonzalo G Islas-Trejo Alma A Wickramasinghe Saumya S Medrano Juan F JF
Mammalian genome : official journal of the International Mammalian Genome Society 20101106 11-12
High-throughput sequencing of RNA (RNA-Seq) was developed primarily to analyze global gene expression in different tissues. However, it also is an efficient way to discover coding SNPs. The objective of this study was to perform a SNP discovery analysis in the milk transcriptome using RNA-Seq. Seven milk samples from Holstein cows were analyzed by sequencing cDNAs using the Illumina Genome Analyzer system. We detected 19,175 genes expressed in milk samples corresponding to approximately 70% of t ...[more]
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