Ontology highlight
ABSTRACT:
SUBMITTER: Pan YS
PROVIDER: S-EPMC3004410 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Pan Yi-Shin YS Wei Hung-Ju HJ Chang Chung-Chieh CC Lin Chung-Hung CH Wei Ting-Shyang TS Wu Suh-Chin SC Chang Ding-Kwo DK
Journal of biomedicine & biotechnology 20101209
We have constructed virus-like particles (VLPs) harboring hemagglutinin (HA), neuraminidase (NA), matrix protein 1 (M1) ,and proton channel protein (M2) using baculovirus as a vector in the SF9 insect cell. The size of the expressed VLP was estimated to be ~100 nm by light scattering experiment and transmission electron microscopy. Recognition of HA on the VLP surface by the HA2-specific monoclonal antibody IIF4 at acidic pH, as probed by surface plasmon resonance, indicated the pH-induced struc ...[more]