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Allosteric regulation of glycogen synthase kinase 3?: a theoretical study.


ABSTRACT: Glycogen synthase kinase 3? (GSK-3?) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3? is regulated via multiple pathways and sites. We performed all-atom molecular dynamics simulations on the unphosphorylated and phosphorylated unbound GSK-3? and the phosphorylated GSK-3? bound to a peptide substrate, its product, and a derived inhibitor. We found that GSK-3? autophosphorylation at residue Tyr(216) results in widening of the catalytic groove, thereby facilitating substrate access. In addition, we studied the interactions of the phosphorylated GSK-3? with a substrate and peptide inhibitor located at the active site and observed higher affinity of the inhibitor to the kinase. Furthermore, we detected a potential remote binding site which was previously identified in other kinases. In agreement with experiments we observed that binding of specific peptides at this remote site leads to stabilization of the activation loop located in the active site. We speculate that this stabilization could enhance the catalytic activity of the kinase. We point to this remote site as being structurally conserved and suggest that the allosteric phenomenon observed here may occur in the protein kinase superfamily.

SUBMITTER: Buch I 

PROVIDER: S-EPMC3005830 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Allosteric regulation of glycogen synthase kinase 3β: a theoretical study.

Buch Idit I   Fishelovitch Dan D   London Nir N   Raveh Barak B   Wolfson Haim J HJ   Nussinov Ruth R  

Biochemistry 20101203 51


Glycogen synthase kinase 3β (GSK-3β) is a serine-threonine kinase belonging to the CMGC family that plays a key role in many biological processes, such as glucose metabolism, cell cycle regulation, and proliferation. Like most protein kinases, GSK-3β is regulated via multiple pathways and sites. We performed all-atom molecular dynamics simulations on the unphosphorylated and phosphorylated unbound GSK-3β and the phosphorylated GSK-3β bound to a peptide substrate, its product, and a derived inhib  ...[more]

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