Analysis of the signaling pathways regulating Src-dependent remodeling of the actin cytoskeleton.

ABSTRACT: Cell adhesion to the extracellular matrix is mediated by adhesion receptors, mainly integrins, which upon interaction with the extracellular matrix, bind to the actin cytoskeleton via their cytoplasmic domains. This association is mediated by a variety of scaffold and signaling proteins, which control the mechanical and signaling activities of the adhesion site. Upon transformation of fibroblasts with active forms of Src (e.g., v-Src), focal adhesions are disrupted, and transformed into dot-like contacts known as podosomes, and consisting of a central actin core surrounded by an adhesion ring. To clarify the mechanism underlying Src-dependent modulation of the adhesive phenotype, and its influence on podosome organization, we screened for the effect of siRNA-mediated knockdown of tyrosine

SUBMITTER: Winograd-Katz SE 

PROVIDER: S-EPMC3005982 | biostudies-literature | 2011 Feb-Mar

REPOSITORIES: biostudies-literature