Identification of synthetic inducers and inhibitors of the quorum-sensing regulator LasR in Pseudomonas aeruginosa by high-throughput screening.
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ABSTRACT:
SUBMITTER: Borlee BR
PROVIDER: S-EPMC3008242 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
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Identification of synthetic inducers and inhibitors of the quorum-sensing regulator LasR in Pseudomonas aeruginosa by high-throughput screening.
Borlee Bradley R BR Geske Grant D GD Blackwell Helen E HE Handelsman Jo J
Applied and environmental microbiology 20101008 24
We report the screening of 16,000 synthetic compounds for induction and inhibition of quorum sensing in a Pseudomonas putida N-acylated l-homoserine lactone (AHL) sensor strain engineered with the LasR transcriptional activator. LasR controls virulence gene expression in the opportunistic pathogen Pseudomonas aeruginosa and is of significant interest as a therapeutic target. Nine compounds that inhibit and 14 compounds that induce LasR activity were identified in our high-throughput screen. ...[more]
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