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Structural basis of the association of HIV-1 matrix protein with DNA.

ABSTRACT: HIV-1 matrix (MA) is a multifunctional protein that is synthesized as a polyprotein that is cleaved by protease during viral maturation. MA contains a cluster of basic residues whose role is controversial. Proposed functions include membrane anchoring, facilitating viral assembly, and directing nuclear import of the viral DNA. Since MA has been reported to be a component of the preintegration complex (PIC), we have used NMR to probe its interaction with other PIC components. We show that MA interacts with DNA and this is likely sufficient to account for its association with the PIC.

SUBMITTER: Cai M

PROVIDER: S-EPMC3009736 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Structural basis of the association of HIV-1 matrix protein with DNA.

Cai Mengli M Huang Ying Y Craigie Robert R Clore G Marius GM

PloS one 20101223 12

HIV-1 matrix (MA) is a multifunctional protein that is synthesized as a polyprotein that is cleaved by protease during viral maturation. MA contains a cluster of basic residues whose role is controversial. Proposed functions include membrane anchoring, facilitating viral assembly, and directing nuclear import of the viral DNA. Since MA has been reported to be a component of the preintegration complex (PIC), we have used NMR to probe its interaction with other PIC components. We show that MA inte ...[more]

PMID: 21203471

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