Human gene targeting by viral vectors.
Ontology highlight
ABSTRACT:
SUBMITTER: Russell DW
PROVIDER: S-EPMC3010411 | biostudies-literature | 1998 Apr
REPOSITORIES: biostudies-literature
Publications
Stable transduction of mammalian cells typically involves random integration of viral vectors by non-homologous recombination. Here we report that vectors based on adeno-associated virus (AAV) can efficiently modify homologous human chromosomal target sequences. Both integrated neomycin phosphotransferase genes and the hypoxanthine phosphoribosyltransferase gene were targeted by AAV vectors. Site-specific genetic modifications could be introduced into approximately 1% of cells, with the highest ...[more]
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