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Enzyme-assisted binary probe for sensitive detection of RNA and DNA.

ABSTRACT: The new enzyme-assisted assay for DNA/RNA detection provides real-time fluorescent signal readout along with low limit of detection and high discrimination power toward a single-base substitution. Requiring only two new unmodified DNA oligonucleotides for the detection of each new analyte, the assay is an efficient tool for low-cost analysis of multiple analytes.

SUBMITTER: Gerasimova YV

PROVIDER: S-EPMC3010920 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Enzyme-assisted binary probe for sensitive detection of RNA and DNA.

Gerasimova Yulia V YV Peck Sadia S Kolpashchikov Dmitry M DM

Chemical communications (Cambridge, England) 20101021 46

The new enzyme-assisted assay for DNA/RNA detection provides real-time fluorescent signal readout along with low limit of detection and high discrimination power toward a single-base substitution. Requiring only two new unmodified DNA oligonucleotides for the detection of each new analyte, the assay is an efficient tool for low-cost analysis of multiple analytes. ...[more]

PMID: 20967321

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