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Use of photoactivation and photobleaching to monitor the dynamic regulation of E-cadherin at the plasma membrane.

ABSTRACT: The dynamic control of E-cadherin is critical for establishing and maintaining cell-cell junctions in epithelial cells. The concentration of E-cadherin molecules at adherens junctions (AJs) is regulated by lateral movement of E-cadherin within the plasma membrane and endocytosis. Here we set out to study the interplay between these processes and their contribution to E-cadherin dynamics. Using photoactivation (PA) and fluorescence recovery after photobleaching (FRAP) we were able to monitor the fate of E-cadherin molecules within the plasma membrane. Our results suggest that the motility of E-cadherin within, and away from, the cell surface are not exclusive or independent mechanisms and there is a fine balance between the two which when perturbed can have dramatic effects on the regulation of AJs.

SUBMITTER: Canel M

PROVIDER: S-EPMC3011267 | biostudies-literature | 2010 Oct-Dec

REPOSITORIES: biostudies-literature

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Use of photoactivation and photobleaching to monitor the dynamic regulation of E-cadherin at the plasma membrane.

Canel Marta M Serrels Alan A Anderson Kurt I KI Frame Margaret C MC Brunton Valerie G VG

Cell adhesion & migration 20101001 4

The dynamic control of E-cadherin is critical for establishing and maintaining cell-cell junctions in epithelial cells. The concentration of E-cadherin molecules at adherens junctions (AJs) is regulated by lateral movement of E-cadherin within the plasma membrane and endocytosis. Here we set out to study the interplay between these processes and their contribution to E-cadherin dynamics. Using photoactivation (PA) and fluorescence recovery after photobleaching (FRAP) we were able to monitor the ...[more]

PMID: 20595808

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