Dataset Information

Expression and function of the K+ channel KCNQ genes in human arteries.

ABSTRACT:

Background and purpose

KCNQ-encoded voltage-gated potassium channels (K(v) 7) have recently been identified as important anti-constrictor elements in rodent blood vessels but the role of these channels and the effects of their modulation in human arteries remain unknown. Here, we have assessed KCNQ gene expression and function in human arteries ex vivo.

Experimental approach

Fifty arteries (41 from visceral adipose tissue, 9 mesenteric arteries) were obtained from subjects undergoing elective surgery. Quantitative RT-PCR experiments using primers specific for all known KCNQ genes and immunohistochemsitry were used to show K(v) 7 channel expression. Wire myography and single cell electrophysiology assessed the function of these channels.

Key results

KCNQ4 was expressed in all arteries assessed, with variable contributions from KCNQ1, 3 and 5. KCNQ2 was not detected. K(v) 7 channel isoform-dependent staining was revealed in the smooth muscle layer. In functional studies, the K(v) 7 channel blockers, XE991 and linopirdine increased isometric tension and inhibited K(+) currents. In contrast, the K(v) 7.1-specific blocker chromanol 293B did not affect vascular tone. Two K(v) 7 channel activators, retigabine and acrylamide S-1, relaxed preconstricted arteries, actions reversed by XE991. K(v) 7 channel activators also suppressed spontaneous contractile activity in seven arteries, reversible by XE991.

Conclusions and implications

This is the first study to demonstrate not only the presence of KCNQ gene products in human arteries but also their contribution to vascular tone ex vivo.

Linked article

This article is commented on by Mani and Byron, pp. 38-41 of this issue. To view this commentary visit http://dx.doi.org/10.1111/j.1476-5381.2010.01065.x.

SUBMITTER: Ng FL

PROVIDER: S-EPMC3012405 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Expression and function of the K+ channel KCNQ genes in human arteries.

Ng Fu Liang FL Davis Alison J AJ Jepps Thomas A TA Harhun Maksym I MI Yeung Shuk Yin SY Wan Andrew A Reddy Marcus M Melville David D Nardi Antonio A Khong Teck K TK Greenwood Iain A IA

British journal of pharmacology 20110101 1

<h4>Background and purpose</h4>KCNQ-encoded voltage-gated potassium channels (K(v) 7) have recently been identified as important anti-constrictor elements in rodent blood vessels but the role of these channels and the effects of their modulation in human arteries remain unknown. Here, we have assessed KCNQ gene expression and function in human arteries ex vivo.<h4>Experimental approach</h4>Fifty arteries (41 from visceral adipose tissue, 9 mesenteric arteries) were obtained from subjects undergo ...[more]

PMID: 20840535

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Dataset Information

Expression and function of the K+ channel KCNQ genes in human arteries.

Background and purpose

Experimental approach

Key results

Conclusions and implications

Linked article

Publications

Expression and function of the K+ channel KCNQ genes in human arteries.

Similar Datasets

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