Membrane protein biosensing with plasmonic nanopore arrays and pore-spanning lipid membranes.

ABSTRACT: Integration of solid-state biosensors and lipid bilayer membranes is important for membrane protein research and drug discovery. In these sensors, it is critical that the solid-state sensing material does not have adverse effects on the conformation or functionality of membrane-bound molecules. In this work, pore-spanning lipid membranes are formed over an array of periodic nanopores in free-standing gold films for surface plasmon resonance (SPR) kinetic binding assays. The ability to perform kinetic assays with a transmembrane protein is demonstrated with ?-hemolysin (?-HL). The incorporation of ?-HL into the membrane followed by specific antibody binding (anti-?-HL) red-shifts the plasmon resonance of the gold nanopore array, which is optically monitored in real time. Subsequent fluorescence imaging reveals that the antibodies primarily bind in nanopore regions, indicating that ?-HL incorporation preferentially occurs into areas of pore-spanning lipid membranes.

SUBMITTER: Im H 

PROVIDER: S-EPMC3015192 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature