Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.
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ABSTRACT:
SUBMITTER: Li J
PROVIDER: S-EPMC3016283 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Publications
Replication and transcription of human papillomavirus type 58 genome in Saccharomyces cerevisiae.
Li Jing J Wang Xiao X Liu Juan J Wang Hong H Zhang Xiao-Li XL Tang Wei W Sun Yun-Dong YD Wang Xin X Yu Xiu-Ping XP Zhao Wei-Ming WM
Virology journal 20101215
<h4>Background</h4>To establish a convenient system for the study of human papillomavirus (HPV), we inserted a Saccharomyces cerevisiae selectable marker, Ura, into HPV58 genome and transformed it into yeast.<h4>Results</h4>HPV58 genome could replicate extrachromosomally in yeast, with transcription of its early and late genes. However, with mutation of the viral E2 gene, HPV58 genome lost its mitotic stability, and the transcription levels of E6 and E7 genes were upregulated.<h4>Conclusions</h4 ...[more]
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