Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii.
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ABSTRACT:
SUBMITTER: Blaby IK
PROVIDER: S-EPMC3017900 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Publications
Towards a systems approach in the genetic analysis of archaea: Accelerating mutant construction and phenotypic analysis in Haloferax volcanii.
Blaby Ian K IK Phillips Gabriela G Blaby-Haas Crysten E CE Gulig Kevin S KS El Yacoubi Basma B de Crécy-Lagard Valérie V
Archaea (Vancouver, B.C.) 20101223
With the availability of a genome sequence and increasingly sophisticated genetic tools, Haloferax volcanii is becoming a model for both Archaea and halophiles. In order for H. volcanii to reach a status equivalent to Escherichia coli, Bacillus subtilis, or Saccharomyces cerevisiae, a gene knockout collection needs to be constructed in order to identify the archaeal essential gene set and enable systematic phenotype screens. A streamlined gene-deletion protocol adapted for potential automation w ...[more]
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