Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum.
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SUBMITTER: Gronlund M
PROVIDER: S-EPMC3018388 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
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Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum.
Grønlund Mette M Olsen Anne A Johansen Elisabeth I EI Jakobsen Iver I
Plant methods 20101214
Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants, which are difficult to transform. The pea early-browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. However, the available PEBV-VIGS protocols are inadequate for studying genes involved in the symbiosis with arbuscular mycorrhizal fungi (AMF).Here we describe a PEBV-VIGS protocol suitable for reverse genetics studies in ...[more]
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