An intein-mediated site-specific click conjugation strategy for improved tumor targeting of nanoparticle systems.

ABSTRACT: The ability to modify and directly target nanoparticulate carriers has greatly increased their applicability in diagnostic and therapeutic studies. Generally essential to the targeting of nanoparticles is the bioconjugation of targeting ligands to the agent's surface. While bioconjugation techniques have steadily improved in recent years, the field is still plagued with inefficient conjugations reactions and/or the lack of site-specific coupling. To overcome these limitations, click chemistry and expressed protein ligation (EPL) are combined to produce a highly efficient, site-specific reaction. This new EPL-click conjugation strategy is applied to create superparamagnetic iron oxide nanoparticles (SPIO) labeled with HER2/neu affibodies. These HER2-SPIO nanoparticles prove to be highly pot

SUBMITTER: Elias DR 

PROVIDER: S-EPMC3019098 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature