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Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check.


ABSTRACT: In Escherichia coli, cytokinesis is orchestrated by FtsZ, which forms a Z-ring to drive septation. Spatial and temporal control of Z-ring formation is achieved by the Min and nucleoid occlusion (NO) systems. Unlike the well-studied Min system, less is known about the anti-DNA guillotining NO process. Here, we describe studies addressing the molecular mechanism of SlmA (synthetic lethal with a defective Min system)-mediated NO. SlmA contains a TetR-like DNA-binding fold, and chromatin immunoprecipitation analyses show that SlmA-binding sites are dispersed on the chromosome except the Ter region, which segregates immediately before septation. SlmA binds DNA and FtsZ simultaneously, and the SlmA-FtsZ structure reveals that two FtsZ molecules sandwich a SlmA dimer. In this complex, FtsZ can still bind GTP and form protofilaments, but the separated protofilaments are forced into an anti-parallel arrangement. This suggests that SlmA may alter FtsZ polymer assembly. Indeed, electron microscopy data, showing that SlmA-DNA disrupts the formation of normal FtsZ polymers and induces distinct spiral structures, supports this. Thus, the combined data reveal how SlmA derails Z-ring formation at the correct place and time to effect NO.

SUBMITTER: Tonthat NK 

PROVIDER: S-EPMC3020112 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Molecular mechanism by which the nucleoid occlusion factor, SlmA, keeps cytokinesis in check.

Tonthat Nam Ky NK   Arold Stefan T ST   Pickering Brian F BF   Van Dyke Michael W MW   Liang Shoudan S   Lu Yue Y   Beuria Tushar K TK   Margolin William W   Schumacher Maria A MA  

The EMBO journal 20101126 1


In Escherichia coli, cytokinesis is orchestrated by FtsZ, which forms a Z-ring to drive septation. Spatial and temporal control of Z-ring formation is achieved by the Min and nucleoid occlusion (NO) systems. Unlike the well-studied Min system, less is known about the anti-DNA guillotining NO process. Here, we describe studies addressing the molecular mechanism of SlmA (synthetic lethal with a defective Min system)-mediated NO. SlmA contains a TetR-like DNA-binding fold, and chromatin immunopreci  ...[more]

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