Ontology highlight
ABSTRACT:
SUBMITTER: Higman VA
PROVIDER: S-EPMC3020303 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Higman Victoria A VA Boyd Jonathan J Smith Lorna J LJ Redfield Christina C
Journal of biomolecular NMR 20101224 1
RDCs for the 14 kDa protein hen egg-white lysozyme (HEWL) have been measured in eight different alignment media. The elongated shape and strongly positively charged surface of HEWL appear to limit the protein to four main alignment orientations. Furthermore, low levels of alignment and the protein's interaction with some alignment media increases the experimental error. Together with heterogeneity across the alignment media arising from constraints on temperature, pH and ionic strength for some ...[more]