Ontology highlight
ABSTRACT:
SUBMITTER: Owen DJ
PROVIDER: S-EPMC302036 | biostudies-literature | 2000 Aug
REPOSITORIES: biostudies-literature
Owen D J DJ Vallis Y Y Pearse B M BM McMahon H T HT Evans P R PR
The EMBO journal 20000801 16
The heterotetrameric AP2 adaptor (alpha, beta 2, mu 2 and sigma 2 subunits) plays a central role in clathrin-mediated endocytosis. We present the protein recruitment function and 1.7 A resolution structure of its beta 2-appendage domain to complement those previously determined for the mu 2 subunit and alpha appendage. Using structure-directed mutagenesis, we demonstrate the ability of the beta 2 appendage alone to bind directly to clathrin and the accessory proteins AP180, epsin and eps15 at th ...[more]