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Observation of a new pattern in serogroup-related PCR typing of Listeria monocytogenes 4b isolates.

ABSTRACT: Molecular serogroup-related PCR typing has made the determination of serotypes of Listeria monocytogenes isolates easy and rapid. Amplification of selected lineage- and serotype-related genes can produce serotype patterns reflecting the four major serotypes, 1/2a, 1/2b, 1/2c, and 4b. We found that four isolates in our routine testing had a pattern with the four bands lmo0737, ORF2110, ORF2819, and prs positive, a pattern which has not been previously reported in the literature. After testing with a lineage-specific PCR, hybridization, and conventional agglutination serotyping, the isolates with the new pattern were considered to be serotype 4b.

SUBMITTER: Huang B

PROVIDER: S-EPMC3020444 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Observation of a new pattern in serogroup-related PCR typing of Listeria monocytogenes 4b isolates.

Huang Bixing B Fang Ningxia N Dimovski Karolina K Wang Xian X Hogg Geoff G Bates John J

Journal of clinical microbiology 20101103 1

Molecular serogroup-related PCR typing has made the determination of serotypes of Listeria monocytogenes isolates easy and rapid. Amplification of selected lineage- and serotype-related genes can produce serotype patterns reflecting the four major serotypes, 1/2a, 1/2b, 1/2c, and 4b. We found that four isolates in our routine testing had a pattern with the four bands lmo0737, ORF2110, ORF2819, and prs positive, a pattern which has not been previously reported in the literature. After testing wit ...[more]

PMID: 21048013

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