Dataset Information

Influence of plant polymers on the distribution and cultivation of bacteria in the phylum Acidobacteria.

ABSTRACT: Members of the phylum Acidobacteria are among the most abundant bacteria in soil. Although they have been characterized as versatile heterotrophs, it is unclear if the types and availability of organic resources influence their distribution in soil. The potential for organic resources to select for different acidobacteria was assessed using molecular and cultivation-based approaches with agricultural and managed grassland soils in Michigan. The distribution of acidobacteria varied with the carbon content of soil: the proportion of subdivision 4 sequences was highest in agricultural soils (ca. 41%) that contained less carbon than grassland soils, where the proportions of subdivision 1, 3, 4, and 6 sequences were similar. Either readily oxidizable carbon or plant polymers were used as the sole carbon and energy source to isolate heterotrophic bacteria from these soils. Plant polymers increased the diversity of acidobacteria cultivated but decreased the total number of heterotrophs recovered compared to readily oxidizable carbon. Two phylogenetically novel Acidobacteria strains isolated on the plant polymer medium were characterized. Strains KBS 83 (subdivision 1) and KBS 96 (subdivision 3) are moderate acidophiles with pH optima of 5.0 and 6.0, respectively. Both strains grew slowly (? = 0.01 h(-1)) and harbored either 1 (strain KBS 83) or 2 (strain KBS 96) copies of the 16S rRNA encoding gene-a genomic characteristic typical of oligotrophs. Strain KBS 83 is a microaerophile, growing optimally at 8% oxygen. These metabolic characteristics help delineate the niches that acidobacteria occupy in soil and are consistent with their widespread distribution and abundance.

SUBMITTER: Eichorst SA

PROVIDER: S-EPMC3020536 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Influence of plant polymers on the distribution and cultivation of bacteria in the phylum Acidobacteria.

Eichorst Stephanie A SA Kuske Cheryl R CR Schmidt Thomas M TM

Applied and environmental microbiology 20101119 2

Members of the phylum Acidobacteria are among the most abundant bacteria in soil. Although they have been characterized as versatile heterotrophs, it is unclear if the types and availability of organic resources influence their distribution in soil. The potential for organic resources to select for different acidobacteria was assessed using molecular and cultivation-based approaches with agricultural and managed grassland soils in Michigan. The distribution of acidobacteria varied with the carbo ...[more]

PMID: 21097594

Similar Datasets

Project description:The heavy use of mineral fertilizers causes imbalances in the biological processes that take place in soil. Therefore, it is necessary to develop more effective fertilizers or fertilizer complexes that ensure agricultural productivity and soil conservation. There is currently a lack of knowledge regarding the effectiveness of biologically enriched, complex mineral fertilizers for spring barley fertilization. The hypothesis of this study was that bacteria-enriched (Paenibacillus azotofixans, Bacillus megaterium, Bacillus mucilaginosus, and Bacillus mycoides), complex mineral fertilizers (N5P20.5K36) have significant impacts on the yield and potential for economic use of spring barley. Experimental studies were carried out for three years (2020-2022) with sandy loam soil in southern Lithuania. Four different spring barley fertilization scenarios (SCs) were investigated. In SC-1 (control), complex mineral fertilizer (N5P20.5K36) was not applied. In the other SCs, spring barley was sown with a drill and fertilizers were incorporated locally during the sowing operation: fertilization scenario SC-2 used 300 kg ha-1, SC-3 used 150 kg ha-1 preceded by a bacteria-inoculated complex mineral fertilizer (N5P20.5K36), and SC-4 used 300 kg ha-1 with the same bacterial complex. The results showed that the bacterial inoculant increased the efficiency of the mineral fertilizer and had an effect on plant growth in barley. For three consecutive years in the same plots, the bacterial inoculant showed significant positive effects on grain yield (changes of 8.1% in 2020, 6.8% in 2021, and 17.3% in 2022 between SC-2 and SC-4). Comparing the several different fertilizer scenarios from an economic point of view, it was observed that the highest profit per hectare was obtained with SC-4 in all three years of the study. Comparing SC-4 and SC-2, an increase of 13.7% was observed in 2020, followed by 9.1% and 41.9% in 2021 and in 2022, respectively. This study will be useful for farmers, biological inoculant manufacturers, and scientists researching the effectiveness of biological inoculants for growing agricultural crops. We found that it is possible to increase the yield of barley (7-17%) using the same rate of mineral fertilization by enriching it with bacterial inoculants. Further studies should be conducted to determine the effects of the bacterial inoculant on crop yield and soil over a period longer than 3 years.

Dataset Information

Influence of plant polymers on the distribution and cultivation of bacteria in the phylum Acidobacteria.

Publications

Influence of plant polymers on the distribution and cultivation of bacteria in the phylum Acidobacteria.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets