Ontology highlight
ABSTRACT:
SUBMITTER: Matern H
PROVIDER: S-EPMC302084 | biostudies-literature | 2000 Sep
REPOSITORIES: biostudies-literature
Matern H H Yang X X Andrulis E E Sternglanz R R Trepte H H HH Gallwitz D D
The EMBO journal 20000901 17
Through two-hybrid interactions, protein affinity and localization studies, we previously identified Yip1p, an integral yeast Golgi membrane protein able to bind the Ras-like GTPases Ypt1p and Ypt31p in their GDP-bound conformation. In a further two-hybrid screen, we identified Yif1p as an interacting factor of Yip1p. We show that Yif1p is an evolutionarily conserved, essential 35.5 kDa transmembrane protein that forms a tight complex with Yip1p on Golgi membranes. The hydrophilic N-terminal hal ...[more]