Ontology highlight
ABSTRACT:
SUBMITTER: Manzo C
PROVIDER: S-EPMC3021660 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Biophysical journal 20110101 2
Characterization of molecular dynamics on living cell membranes at the nanoscale is fundamental to unravel the mechanisms of membrane organization and compartmentalization. Here we demonstrate the feasibility of fluorescence correlation spectroscopy (FCS) based on the nanometric illumination of near-field scanning optical microscopy (NSOM) probes on intact living cells. NSOM-FCS applied to fluorescent lipid analogs allowed us to reveal details of the diffusion hidden by larger illumination areas ...[more]