Dataset Information

Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2.

ABSTRACT: Human digestive carboxypeptidases CPA1, CPA2, and CPB1 are secreted by the pancreas as inactive proenzymes containing a 94-96-amino acid-long propeptide. Activation of procarboxypeptidases is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin. Here, we demonstrate that subsequent cleavage of the propeptide by chymotrypsin C (CTRC) induces a nearly 10-fold increase in the activity of trypsin-activated CPA1 and CPA2, whereas CPB1 activity is unaffected. Other human pancreatic proteases such as chymotrypsin B1, chymotrypsin B2, chymotrypsin-like enzyme-1, elastase 2A, elastase 3A, or elastase 3B are inactive or markedly less effective at promoting procarboxypeptidase activation. On the basis of these observations, we propose that CTRC is a physiological co-activator of proCPA1 and proCPA2. Furthermore, the results confirm and extend the notion that CTRC is a key regulator of digestive zymogen activation.

SUBMITTER: Szmola R

PROVIDER: S-EPMC3023477 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2.

Szmola Richárd R Bence Melinda M Carpentieri Andrea A Szabó András A Costello Catherine E CE Samuelson John J Sahin-Tóth Miklós M

The Journal of biological chemistry 20101122 3

Human digestive carboxypeptidases CPA1, CPA2, and CPB1 are secreted by the pancreas as inactive proenzymes containing a 94-96-amino acid-long propeptide. Activation of procarboxypeptidases is initiated by proteolytic cleavage at the C-terminal end of the propeptide by trypsin. Here, we demonstrate that subsequent cleavage of the propeptide by chymotrypsin C (CTRC) induces a nearly 10-fold increase in the activity of trypsin-activated CPA1 and CPA2, whereas CPB1 activity is unaffected. Other huma ...[more]

PMID: 21098023

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Dataset Information

Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2.

Publications

Chymotrypsin C is a co-activator of human pancreatic procarboxypeptidases A1 and A2.

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