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Serious complications in gene-expression studies with stress perturbation: An example of UV-exposed p53-mutant mouse embryonic fibroblasts.

ABSTRACT: Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design.

SUBMITTER: Bruning O 

PROVIDER: S-EPMC3023578 | biostudies-literature | 2010 Nov

REPOSITORIES: biostudies-literature

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Serious complications in gene-expression studies with stress perturbation: An example of UV-exposed p53-mutant mouse embryonic fibroblasts.

Bruning Oskar O   Yuan Xiaolian X   Rodenburg Wendy W   Bruins Wendy W   van Oostrom Conny T CT   Rauwerda Han H   Wittink Floyd Ra FR   Jonker Martijs J MJ   de Vries Annemieke A   Breit Timo M TM  

Transcription 20100830 3

Reanalysis of our UV study of p53-mutant mouse embryonic fibroblasts revealed an intriguing orchestration of massive transcriptome responses. However, close scrutiny of the data uncovered an affected mRNA/rRNA ratio, effectively inhibiting valid data analysis. UV-dose range-finding showed low-dose UV specific- and high-dose stress-related responses, which represent a plea for UV dose range-finding in experimental design. ...[more]

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