Quantitative analysis of the efficiency and mutagenic spectra of abasic lesion bypass catalyzed by human Y-family DNA polymerases.

ABSTRACT: Higher eukaryotes encode various Y-family DNA polymerases to perform global DNA lesion bypass. To provide complete mutation spectra for abasic lesion bypass, we employed short oligonucleotide sequencing assays to determine the sequences of abasic lesion bypass products synthesized by human Y-family DNA polymerases eta (hPol?), iota (hPol?) and kappa (hPol?). The fourth human Y-family DNA polymerase, Rev1, failed to generate full-length lesion bypass products after 3?h. The results indicate that hPol? generates mutations with a frequency from 10 to 80% during each nucleotide incorporation event. In contrast, hPol? is the least error prone, generating the fewest mutations in the vicinity of the abasic lesion and inserting dAMP with a frequency of 67% opposite the abasic site. While the error frequency of hPol? is intermediate to those of hPol? and hPol?, hPol? has the highest potential to create frameshift mutations opposite the abasic site. Moreover, the time (t(50)(bypass)) required to bypass 50% of the abasic lesions encountered by hPol?, hPol? and hPol? was 4.6, 112 and 1?823?s, respectively. These t(50)(bypass) values indicate that, among the enzymes, hPol? has the highest abasic lesion bypass efficiency. Together, our data suggest that hPol? is best suited to perform abasic lesion bypass in vivo.

SUBMITTER: Sherrer SM 

PROVIDER: S-EPMC3025555 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature