Ontology highlight
ABSTRACT:
SUBMITTER: Johansson H
PROVIDER: S-EPMC3025563 | biostudies-literature | 2011 Jan
REPOSITORIES: biostudies-literature
Johansson H H Isaksson M M Sörqvist E Falk EF Roos F F Stenberg J J Sjöblom T T Botling J J Micke P P Edlund K K Fredriksson S S Kultima H Göransson HG Ericsson Olle O Nilsson Mats M
Nucleic acids research 20101108 2
Targeted genome enrichment is a powerful tool for making use of the massive throughput of novel DNA-sequencing instruments. We herein present a simple and scalable protocol for multiplex amplification of target regions based on the Selector technique. The updated version exhibits improved coverage and compatibility with next-generation-sequencing (NGS) library-construction procedures for shotgun sequencing with NGS platforms. To demonstrate the performance of the technique, all 501 exons from 28 ...[more]