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Label-free, arrayed sensing of immune response to influenza antigens.

ABSTRACT: Periodic outbreaks of pandemic influenza have been a devastating cause of human mortality over the past century. More recently, an avian influenza strain, designated H5N1, has been identified as having the potential to cause a zoogenic pandemic in humans, and a current outbreak of a new H1N1 influenza variant hypothesized to be of swine origin is of considerable concern. In order to facilitate surveillance and the rapid assessment and comparison of vaccination efforts, a high-throughput assay is highly desirable to supplement standard methods, which require high biosafety-level facilities. In this paper, we describe the design, production, and preliminary evaluation of an antigen array incorporating a panel of hemagglutinins as a platform for the detection and rapid quantification of influenza-specific antibodies in human serum by Arrayed Imaging Reflectometry (AIR), a label-free optical biosensor.

SUBMITTER: Mace CR

PROVIDER: S-EPMC3026320 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Label-free, arrayed sensing of immune response to influenza antigens.

Mace Charles R CR Topham David J DJ Mosmann Tim R TR Quataert Sally A SA Treanor John J JJ Miller Benjamin L BL

Talanta 20101110 3

Periodic outbreaks of pandemic influenza have been a devastating cause of human mortality over the past century. More recently, an avian influenza strain, designated H5N1, has been identified as having the potential to cause a zoogenic pandemic in humans, and a current outbreak of a new H1N1 influenza variant hypothesized to be of swine origin is of considerable concern. In order to facilitate surveillance and the rapid assessment and comparison of vaccination efforts, a high-throughput assay is ...[more]

PMID: 21147350

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