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Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution.

ABSTRACT: Mycobacteriophages are viruses that infect mycobacterial hosts such as Mycobacterium smegmatis and Mycobacterium tuberculosis. All mycobacteriophages characterized to date are dsDNA tailed phages, and have either siphoviral or myoviral morphotypes. However, their genetic diversity is considerable, and although sixty-two genomes have been sequenced and comparatively analyzed, these likely represent only a small portion of the diversity of the mycobacteriophage population at large. Here we report the isolation, sequencing and comparative genomic analysis of 18 new mycobacteriophages isolated from geographically distinct locations within the United States. Although no clear correlation between location and genome type can be discerned, these genomes expand our knowledge of mycobacteriophage diversity and enhance our understanding of the roles of mobile elements in viral evolution. Expansion of the number of mycobacteriophages grouped within Cluster A provides insights into the basis of immune specificity in these temperate phages, and we also describe a novel example of apparent immunity theft. The isolation and genomic analysis of bacteriophages by freshman college students provides an example of an authentic research experience for novice scientists.

SUBMITTER: Pope WH

PROVIDER: S-EPMC3029335 | biostudies-literature | 2011 Jan

REPOSITORIES: biostudies-literature

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Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution.

Pope Welkin H WH Jacobs-Sera Deborah D Russell Daniel A DA Peebles Craig L CL Al-Atrache Zein Z Alcoser Turi A TA Alexander Lisa M LM Alfano Matthew B MB Alford Samantha T ST Amy Nichols E NE Anderson Marie D MD Anderson Alexander G AG Ang Andrew A S AA Ares Manuel M Barber Amanda J AJ Barker Lucia P LP Barrett Jonathan M JM Barshop William D WD Bauerle Cynthia M CM Bayles Ian M IM Belfield Katherine L KL Best Aaron A AA Borjon Agustin A Bowman Charles A CA Boyer Christine A CA Bradley Kevin W KW Bradley Victoria A VA Broadway Lauren N LN Budwal Keshav K Busby Kayla N KN Campbell Ian W IW Campbell Anne M AM Carey Alyssa A Caruso Steven M SM Chew Rebekah D RD Cockburn Chelsea L CL Cohen Lianne B LB Corajod Jeffrey M JM Cresawn Steven G SG Davis Kimberly R KR Deng Lisa L Denver Dee R DR Dixon Breyon R BR Ekram Sahrish S Elgin Sarah C R SC Engelsen Angela E AE English Belle E V BE Erb Marcella L ML Estrada Crystal C Filliger Laura Z LZ Findley Ann M AM Forbes Lauren L Forsyth Mark H MH Fox Tyler M TM Fritz Melissa J MJ Garcia Roberto R George Zindzi D ZD Georges Anne E AE Gissendanner Christopher R CR Goff Shannon S Goldstein Rebecca R Gordon Kobie C KC Green Russell D RD Guerra Stephanie L SL Guiney-Olsen Krysta R KR Guiza Bridget G BG Haghighat Leila L Hagopian Garrett V GV Harmon Catherine J CJ Harmson Jeremy S JS Hartzog Grant A GA Harvey Samuel E SE He Siping S He Kevin J KJ Healy Kaitlin E KE Higinbotham Ellen R ER Hildebrandt Erin N EN Ho Jason H JH Hogan Gina M GM Hohenstein Victoria G VG Holz Nathan A NA Huang Vincent J VJ Hufford Ericka L EL Hynes Peter M PM Jackson Arrykka S AS Jansen Erica C EC Jarvik Jonathan J Jasinto Paul G PG Jordan Tuajuanda C TC Kasza Tomas T Katelyn Murray A MA Kelsey Jessica S JS Kerrigan Larisa A LA Khaw Daryl D Kim Junghee J Knutter Justin Z JZ Ko Ching-Chung CC Larkin Gail V GV Laroche Jennifer R JR Latif Asma A Leuba Kohana D KD Leuba Sequoia I SI Lewis Lynn O LO Loesser-Casey Kathryn E KE Long Courtney A CA Lopez A Javier AJ Lowery Nicholas N Lu Tina Q TQ Mac Victor V Masters Isaac R IR McCloud Jazmyn J JJ McDonough Molly J MJ Medenbach Andrew J AJ Menon Anjali A Miller Rachel R Morgan Brandon K BK Ng Patrick C PC Nguyen Elvis E Nguyen Katrina T KT Nguyen Emilie T ET Nicholson Kaylee M KM Parnell Lindsay A LA Peirce Caitlin E CE Perz Allison M AM Peterson Luke J LJ Pferdehirt Rachel E RE Philip Seegren V SV Pogliano Kit K Pogliano Joe J Polley Tamsen T Puopolo Erica J EJ Rabinowitz Hannah S HS Resiss Michael J MJ Rhyan Corwin N CN Robinson Yetta M YM Rodriguez Lauren L LL Rose Andrew C AC Rubin Jeffrey D JD Ruby Jessica A JA Saha Margaret S MS Sandoz James W JW Savitskaya Judith J Schipper Dale J DJ Schnitzler Christine E CE Schott Amanda R AR Segal J Bradley JB Shaffer Christopher D CD Sheldon Kathryn E KE Shepard Erica M EM Shepardson Jonathan W JW Shroff Madav K MK Simmons Jessica M JM Simms Erika F EF Simpson Brandy M BM Sinclair Kathryn M KM Sjoholm Robert L RL Slette Ingrid J IJ Spaulding Blaire C BC Straub Clark L CL Stukey Joseph J Sughrue Trevor T Tang Tin-Yun TY Tatyana Lyons M LM Taylor Stephen B SB Taylor Barbara J BJ Temple Louise M LM Thompson Jasper V JV Tokarz Michael P MP Trapani Stephanie E SE Troum Alexander P AP Tsay Jonathan J Tubbs Anthony T AT Walton Jillian M JM Wang Danielle H DH Wang Hannah H Warner John R JR Weisser Emilie G EG Wendler Samantha C SC Weston-Hafer Kathleen A KA Whelan Hilary M HM Williamson Kurt E KE Willis Angelica N AN Wirtshafter Hannah S HS Wong Theresa W TW Wu Phillip P Yang Yun jeong Yj Yee Brandon C BC Zaidins David A DA Zhang Bo B Zúniga Melina Y MY Hendrix Roger W RW Hatfull Graham F GF

PloS one 20110127 1

Mycobacteriophages are viruses that infect mycobacterial hosts such as Mycobacterium smegmatis and Mycobacterium tuberculosis. All mycobacteriophages characterized to date are dsDNA tailed phages, and have either siphoviral or myoviral morphotypes. However, their genetic diversity is considerable, and although sixty-two genomes have been sequenced and comparatively analyzed, these likely represent only a small portion of the diversity of the mycobacteriophage population at large. Here we report ...[more]

PMID: 21298013

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Dataset Information

Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution.

Publications

Expanding the diversity of mycobacteriophages: insights into genome architecture and evolution.

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