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N-acylethanolamines as novel alcohol dehydrogenase 3 substrates.

ABSTRACT: N-acylethanolamines (NAEs) are members of the fatty acid amide family. The NAEs have been proposed to serve as metabolic precursors to N-acylglycines (NAGs). The sequential oxidation of the NAEs by an alcohol dehydrogenase and an aldehyde dehydrogenase would yield the N-acylglycinals and/or the NAGs. Alcohol dehydrogenase 3 (ADH3) is one enzyme that might catalyze this reaction. To define a potential role for ADH3 in NAE catabolism, we synthesized a set of NAEs and evaluated these as ADH3 substrates. NAEs were oxidized by ADH3, yielding the N-acylglycinals as the product. The (V/K)(app) values for the NAEs included here were low relative to cinnamyl alcohol. Our data show that the NAEs can serve as alcohol dehydrogenase substrates.

SUBMITTER: Ivkovic M

PROVIDER: S-EPMC3030644 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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N-acylethanolamines as novel alcohol dehydrogenase 3 substrates.

Ivkovic Milena M Dempsey Daniel R DR Handa Sumit S Hilton Joshua H JH Lowe Edward W EW Merkler David J DJ

Archives of biochemistry and biophysics 20101206 2

N-acylethanolamines (NAEs) are members of the fatty acid amide family. The NAEs have been proposed to serve as metabolic precursors to N-acylglycines (NAGs). The sequential oxidation of the NAEs by an alcohol dehydrogenase and an aldehyde dehydrogenase would yield the N-acylglycinals and/or the NAGs. Alcohol dehydrogenase 3 (ADH3) is one enzyme that might catalyze this reaction. To define a potential role for ADH3 in NAE catabolism, we synthesized a set of NAEs and evaluated these as ADH3 substr ...[more]

PMID: 21144815

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