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CAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.

ABSTRACT: The intracellular second messenger cAMP is frequently used in induction media to induce mesenchymal stem cells (MSCs) into neural lineage cells. To date, an understanding of the role cAMP exerts on MSCs and whether cAMP can induce MSCs into functional neurons is still lacking. We found cAMP initiated neuron-like morphology changes early and neural differentiation much later. The early phase changes in morphology were due to cell shrinkage, which subsequently rendered some cells apoptotic. While the morphology changes occurred prior to the expression of neural markers, it is not required for neural marker expression and the two processes are differentially regulated downstream of cAMP-activated protein kinase A. cAMP enabled MSCs to gain neural marker expressions with neuronal function, such as, calcium rise in response to neuronal activators, dopamine, glutamate, and potassium chloride. However, only some of the cells induced by cAMP responded to the three neuronal activators and further lack the neuronal morphology, suggesting that although cAMP is able to direct MSCs towards neural differentiation, they do not achieve terminal differentiation.

SUBMITTER: Zhang L

PROVIDER: S-EPMC3030935 | biostudies-literature | 2011 Mar

REPOSITORIES: biostudies-literature

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cAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.

Zhang Linxia L Seitz Linsey C LC Abramczyk Amy M AM Liu Li L Chan Christina C

Cellular and molecular life sciences : CMLS 20100820 5

The intracellular second messenger cAMP is frequently used in induction media to induce mesenchymal stem cells (MSCs) into neural lineage cells. To date, an understanding of the role cAMP exerts on MSCs and whether cAMP can induce MSCs into functional neurons is still lacking. We found cAMP initiated neuron-like morphology changes early and neural differentiation much later. The early phase changes in morphology were due to cell shrinkage, which subsequently rendered some cells apoptotic. While ...[more]

PMID: 20725762

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Dataset Information

CAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.

Publications

cAMP initiates early phase neuron-like morphology changes and late phase neural differentiation in mesenchymal stem cells.

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