Ontology highlight
ABSTRACT:
SUBMITTER: Huang CJ
PROVIDER: S-EPMC3030953 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Huang Chang-jun CJ Zhang Tong T Li Fang-fang FF Zhang Xin-yue XY Zhou Xue-ping XP
Journal of Zhejiang University. Science. B 20110201 2
Virus-induced gene silencing (VIGS) is a recently developed technique for characterizing the function of plant genes by gene transcript suppression and is increasingly used to generate transient loss-of-function assays. Here we report that the 2mDNA1, a geminivirus satellite vector, can induce efficient gene silencing in Nicotiana tabacum with Tobacco curly shoot virus. We have successfully silenced the β-glucuronidase (GUS) gene in GUS transgenic N. tabacum plants and the sulphur desaturase (Su ...[more]