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Polarized myosin produces unequal-size daughters during asymmetric cell division.

ABSTRACT: Asymmetric positioning of the mitotic spindle before cytokinesis can produce different-sized daughter cells that have distinct fates. Here, we found an asymmetric division in the Caenorhabditis elegans Q neuroblast lineage that began with a centered spindle but generated different-sized daughters, the smaller (anterior) of which underwent apoptosis. During this division, more myosin II accumulated anteriorly, suggesting that asymmetric contractile forces might produce different-sized daughters. Indeed, partial inactivation of anterior myosin by chromophore-assisted laser inactivation created a more symmetric division and allowed the survival and differentiation of the anterior daughter. Thus, the balance of myosin activity on the two sides of a dividing cell can govern the size and fate of the daughters.

SUBMITTER: Ou G

PROVIDER: S-EPMC3032534 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature

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Polarized myosin produces unequal-size daughters during asymmetric cell division.

Ou Guangshuo G Stuurman Nico N D'Ambrosio Michael M Vale Ronald D RD

Science (New York, N.Y.) 20100930 6004

Asymmetric positioning of the mitotic spindle before cytokinesis can produce different-sized daughter cells that have distinct fates. Here, we found an asymmetric division in the Caenorhabditis elegans Q neuroblast lineage that began with a centered spindle but generated different-sized daughters, the smaller (anterior) of which underwent apoptosis. During this division, more myosin II accumulated anteriorly, suggesting that asymmetric contractile forces might produce different-sized daughters. ...[more]

PMID: 20929735

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