Ontology highlight
ABSTRACT:
SUBMITTER: Doud EH
PROVIDER: S-EPMC3033458 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Doud Emma H EH Perlstein Deborah L DL Wolpert Manuel M Cane David E DE Walker Suzanne S
Journal of the American Chemical Society 20110107 5
The reactions of two bacterial TIM barrel prenyltransferases (PTs), MoeO5 and PcrB, were explored. MoeO5, the enzyme responsible for the first step in moenomycin biosynthesis, catalyzes the transfer of farnesyl to 3-phosphoglyceric acid (3PG) to give a product containing a cis-allylic double bond. We show that this reaction involves isomerization to a nerolidyl pyrophosphate intermediate followed by bond rotation prior to attack by the nucleophile. This mechanism is unprecedented for a prenyltra ...[more]