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Isolation of bacterial ribosomes with monolith chromatography.

ABSTRACT: We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could accelerate the study of many different aspects of ribosomal biology.

SUBMITTER: Trauner A

PROVIDER: S-EPMC3033897 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Isolation of bacterial ribosomes with monolith chromatography.

Trauner Andrej A Bennett Mark H MH Williams Huw D HD

PloS one 20110204 2

We report the development of a rapid chromatographic method for the isolation of bacterial ribosomes from crude cell lysates in less than ten minutes. Our separation is based on the use of strong anion exchange monolithic columns. Using a simple stepwise elution program we were able to purify ribosomes whose composition is comparable to those isolated by sucrose gradient ultracentrifugation, as confirmed by quantitative proteomic analysis (iTRAQ). The speed and simplicity of this approach could ...[more]

PMID: 21326610

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