Influence of histatin 5 on Candida albicans mitochondrial protein expression assessed by quantitative mass spectrometry.

ABSTRACT: Individual aspects of the mode of action of histatin 5, a human salivary antifungal protein, have been partially elucidated, but the mechanism likely involves a complex set of events that have not been characterized. Previous evidence points toward histatin-induced alterations in mitochondrial function. The purpose of the present study was to verify and quantify changes in the mitochondrial proteome of Candida albicans treated with histatin 5. Cell killing was determined by plating and differential protein expression levels in the mitochondrial samples were determined by quantitative proteomics approaches employing mTRAQ and ICAT labeling and Western blotting. Relative quantitation ratios were established for 144 different proteins. Up-regulated mitochondrial proteins were predominantly in

SUBMITTER: Komatsu T 

PROVIDER: S-EPMC3033980 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature