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Crystallization and preliminary crystallographic studies of a cysteine protease inhibitor from the human nematode parasite Ascaris lumbricoides.

ABSTRACT: The cysteine protease inhibitor from Ascaris lumbricoides, a roundworm that lives in the human intestine, may be involved in the suppression of human immune responses. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of the cysteine protease inhibitor from A. lumbricoides are reported. The rod-shaped crystal belonged to space group C2, with unit-cell parameters a = 99.40, b = 37.52, c = 62.92?Å, ? = 118.26°. The crystal diffracted to 2.1?Å resolution and contained two molecules in the asymmetric unit.

SUBMITTER: Liu S

PROVIDER: S-EPMC3034614 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Crystallization and preliminary crystallographic studies of a cysteine protease inhibitor from the human nematode parasite Ascaris lumbricoides.

Liu Sanling S Dong Jianmei J Mei Guoqiang G Liu Guiyun G Xu Wei W Su Zhong Z Liu Jinsong J

Acta crystallographica. Section F, Structural biology and crystallization communications 20110122 Pt 2

The cysteine protease inhibitor from Ascaris lumbricoides, a roundworm that lives in the human intestine, may be involved in the suppression of human immune responses. Here, the molecular cloning, protein expression and purification, preliminary crystallization and crystallographic characterization of the cysteine protease inhibitor from A. lumbricoides are reported. The rod-shaped crystal belonged to space group C2, with unit-cell parameters a = 99.40, b = 37.52, c = 62.92 Å, β = 118.26°. The c ...[more]

PMID: 21301092

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