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The core-independent promoter-specific interaction of primary sigma factor.

ABSTRACT: Previous studies have led to a model in which the promoter-specific recognition of prokaryotic transcription initiation factor, sigma (?), is core dependent. Most ? functions were studied on the basis of this tenet. Here, we provide in vitro evidence demonstrating that the intact Bacillus subtilis primary sigma, ?(A), by itself, is able to interact specifically with promoter deoxyribonucleic acid (DNA), albeit with low sequence selectivity. The core-independent promoter-specific interaction of the ?(A) is -10 specific. However, the promoter -10 specific interaction is unable to allow the ?(A) to discern the optimal promoter spacing. To fulfill this goal, the ?(A) requires assistance from core RNA polymerase (RNAP). The ability of ?, by itself, to interact specifically with promoter might introduce a critical new dimension of study in prokaryotic ? function.

SUBMITTER: Yeh HY

PROVIDER: S-EPMC3035472 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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The core-independent promoter-specific interaction of primary sigma factor.

Yeh Hsin-Yi HY Chen Tsung-Ching TC Liou Kung-Ming KM Hsu Hsiu-Ting HT Chung Kuei-Min KM Hsu Li-Ling LL Chang Ban-Yang BY

Nucleic acids research 20101008 3

Previous studies have led to a model in which the promoter-specific recognition of prokaryotic transcription initiation factor, sigma (σ), is core dependent. Most σ functions were studied on the basis of this tenet. Here, we provide in vitro evidence demonstrating that the intact Bacillus subtilis primary sigma, σ(A), by itself, is able to interact specifically with promoter deoxyribonucleic acid (DNA), albeit with low sequence selectivity. The core-independent promoter-specific interaction of t ...[more]

PMID: 20935043

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