Conversion of barley SNPs into PCR-based markers using dCAPS method.
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ABSTRACT:
SUBMITTER: Shahinnia F
PROVIDER: S-EPMC3036059 | biostudies-literature | 2009 Jul
REPOSITORIES: biostudies-literature
Publications
Conversion of barley SNPs into PCR-based markers using dCAPS method.
Shahinnia Fahimeh F Sayed-Tabatabaei Badraldin Ebrahim BE
Genetics and molecular biology 20090701 3
Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the ...[more]
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