Dataset Information

Dendritic calcium signaling in ON and OFF mouse retinal ganglion cells.

ABSTRACT: Retinal ganglion cells (RGCs) are the output cells of the retina; they convert synaptic input into spike output that carries visual information to the brain. Synaptic inputs are received, integrated and communicated to the spike initiation zone of the axon by dendrites whose properties are poorly understood. Here simultaneous patch-clamp recording and 2-photon Ca(2+) imaging are used to study voltage- and light-evoked Ca(2+) signals in the dendrites of identified types of mouse RGCs from parallel ON and OFF pathways, which encode the onset and offset of light, respectively. The results show pathway-specific differences in voltage-dependent Ca(2+) signaling. While both ON and OFF cells express high-voltage-activated (HVA) Ca(2+) channels, only OFF RGCs also express low-voltage-activated (LVA) Ca(2+) channels. LVA Ca(2+) channels in OFF cells are deinactivated by hyperpolarization from the resting potential and give rise to rebound excited Ca(2+) spikes at the termination of a step of either hyperpolarizing current or light. This suggests that the differential expression of voltage-gated Ca(2+) channels in ON and OFF RGC dendrites contributes to differences in the way the two cell types process visual stimuli.

SUBMITTER: Margolis DJ

PROVIDER: S-EPMC3037260 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Dendritic calcium signaling in ON and OFF mouse retinal ganglion cells.

Margolis David J DJ Gartland Andrew J AJ Euler Thomas T Detwiler Peter B PB

The Journal of neuroscience : the official journal of the Society for Neuroscience 20100501 21

Retinal ganglion cells (RGCs) are the output cells of the retina; they convert synaptic input into spike output that carries visual information to the brain. Synaptic inputs are received, integrated and communicated to the spike initiation zone of the axon by dendrites whose properties are poorly understood. Here simultaneous patch-clamp recording and 2-photon Ca(2+) imaging are used to study voltage- and light-evoked Ca(2+) signals in the dendrites of identified types of mouse RGCs from paralle ...[more]

PMID: 20505081

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Project description:A small number (<2%) of mammalian retinal ganglion cells express the photopigment melanopsin and are intrinsically photosensitive (ipRGCs). Light depolarizes ipRGCs and increases intracellular calcium levels ([Ca2+]i) but the signaling cascades underlying these responses have yet to be elucidated. To facilitate physiological studies on these rare photoreceptors, highly enriched ipRGC cultures from neonatal rats were generated using anti-melanopsin-mediated plate adhesion (immunopanning). This novel approach enabled experiments on isolated ipRGCs, eliminating the potential confounding influence of rod/cone-driven input. Light induced a rise in [Ca2+]i (monitored using fura-2 imaging) in the immunopanned ipRGCs and the source of this Ca2+ signal was investigated. The Ca2+ responses were inhibited by 2-aminoethoxydiphenyl borate, SKF-96365 (1-2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl-1H-imidazole), flufenamic acid, lanthanum, and gadolinium, consistent with the involvement of canonical transient receptor potential (TRP) channels in ipRGC phototransduction. However, the contribution of direct Ca2+ flux through a putative TRP channel to ipRGC [Ca2+]i was relatively small, as most (approximately 90%) of the light-induced Ca2+ responses could be blocked by preventing action potential firing with tetrodotoxin. The L-type voltage-gated Ca2+ channel (VGCC) blockers verapamil and (+)-cis-diltiazem significantly reduced the light-evoked Ca2+ responses, while the internal Ca2+ stores depleting agent thapsigargin had negligible effect. These results indicate that Ca2+ influx through VGCCs, activated after action potential firing, was the primary source for light-evoked elevations in ipRGC [Ca2+]i. Furthermore, concurrent Ca2+ imaging and cell-attached electrophysiological recordings demonstrated that the Ca2+ responses were highly correlated to spike frequency, thereby establishing a direct link between action potential firing and somatic [Ca2+]i in light-stimulated ipRGCs.

Dataset Information

Dendritic calcium signaling in ON and OFF mouse retinal ganglion cells.

Publications

Dendritic calcium signaling in ON and OFF mouse retinal ganglion cells.

Similar Datasets

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