Ontology highlight
ABSTRACT:
SUBMITTER: Ho M
PROVIDER: S-EPMC3038457 | biostudies-literature | 2011 Mar
REPOSITORIES: biostudies-literature
Protein engineering, design & selection : PEDS 20101104 3
The long half-life of botulinum neurotoxin serotype A (BoNT/A) in cells poses a challenge in developing post-exposure therapeutics complementary to existing antitoxin strategies. Delivery vehicles consisting of the toxin heavy chain (HC), including the receptor-binding domain and translocation domain, connected to an inhibitory cargo offer a possible solution for rescuing intoxicated neurons in victims paralyzed from botulism. Here, we report the expression and purification of soluble recombinan ...[more]