Ontology highlight
ABSTRACT:
SUBMITTER: Dave RH
PROVIDER: S-EPMC3039364 | biostudies-literature | 2011 Feb
REPOSITORIES: biostudies-literature
Davé Rahul H RH Saengsawang Witchuda W Lopus Manu M Davé Sonya S Wilson Leslie L Rasenick Mark M MM
The Journal of biological chemistry 20101126 6
The heterotrimeric, G protein-coupled receptor-associated G protein, Gα(s), binds tubulin with nanomolar affinity and disrupts microtubules in cells and in vitro. Here we determine that the activated form of Gα(s) binds tubulin with a K(D) of 100 nm, stimulates tubulin GTPase, and promotes microtubule dynamic instability. Moreover, the data reveal that the α3-β5 region of Gα(s) is a functionally important motif in the Gα(s)-mediated microtubule destabilization. Indeed, peptides corresponding to ...[more]