Ontology highlight
ABSTRACT:
SUBMITTER: Zhao JX
PROVIDER: S-EPMC3039938 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Zhao Jian-Xin JX Yang Lian L Gu Zhen-Nan ZN Chen Hai-Qin HQ Tian Feng-Wei FW Chen Yong-Quan YQ Zhang Hao H Chen Wei W
International journal of molecular sciences 20101223 1
The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4-an anti-aflatoxin B(1) (AFB(1)) scFv-with an interdomain disulfide bond and studied the effect of the disulfide bond on antibody affinity. With homology modeling and molecular docking, we designed a scFv containing an interdomain disulfide bond between the residues H44 and L100. The stability of scFv (H4) increased from a Gdn ...[more]