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Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays.


ABSTRACT: Genetic screening of the breast and ovarian cancer susceptibility gene BRCA1 has uncovered a large number of variants of uncertain clinical significance. Here, we use biochemical and cell-based transcriptional assays to assess the structural and functional defects associated with a large set of 117 distinct BRCA1 missense variants within the essential BRCT domain of the BRCA1 protein that have been documented in individuals with a family history of breast or ovarian cancer. In the first method, we used limited proteolysis to assess the protein folding stability of each of the mutants compared with the wild-type. In the second method, we used a phosphopeptide pull-down assay to assess the ability of each of the variants to specifically interact with a peptide containing a pSer-X-X-Phe motif, a known functional target of the BRCA1 BRCT domain. Finally, we used transcriptional assays to assess the ability of each BRCT variant to act as a transcriptional activation domain in human cells. Through a correlation of the assay results with available family history and clinical data, we define limits to predict the disease risk associated with each variant. Forty-two of the variants show little effect on function and are likely to represent variants with little or no clinical significance; 50 display a clear functional effect and are likely to represent pathogenic variants; and the remaining 25 variants display intermediate activities. The excellent agreement between the structure/function effects of these mutations and available clinical data supports the notion that functional and structure information can be useful in the development of models to assess cancer risk.

SUBMITTER: Lee MS 

PROVIDER: S-EPMC3040717 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Comprehensive analysis of missense variations in the BRCT domain of BRCA1 by structural and functional assays.

Lee Megan S MS   Green Ruth R   Marsillac Sylvia M SM   Coquelle Nicolas N   Williams R Scott RS   Yeung Telford T   Foo Desmond D   Hau D Duong DD   Hui Ben B   Monteiro Alvaro N A AN   Glover J N Mark JN  

Cancer research 20100601 12


Genetic screening of the breast and ovarian cancer susceptibility gene BRCA1 has uncovered a large number of variants of uncertain clinical significance. Here, we use biochemical and cell-based transcriptional assays to assess the structural and functional defects associated with a large set of 117 distinct BRCA1 missense variants within the essential BRCT domain of the BRCA1 protein that have been documented in individuals with a family history of breast or ovarian cancer. In the first method,  ...[more]

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