Dataset Information

Divergence of AMP Deaminase in the Ice Worm Mesenchytraeus solifugus (Annelida, Clitellata, Enchytraeidae).

ABSTRACT: Glacier ice worms, Mesenchytraeus solifugus and related species, are the largest glacially obligate metazoans. As one component of cold temperature adaptation, ice worms maintain atypically high energy levels in an apparent mechanism to offset cold temperature-induced lethargy and death. To explore this observation at a mechanistic level, we considered the putative contribution of 5' adenosine monophosphate deaminase (AMPD), a key regulator of energy metabolism in eukaryotes. We cloned cDNAs encoding ice worm AMPD, generating a fragment encoding 543 amino acids that included a short N-terminal region and complete C-terminal catalytic domain. The predicted ice worm AMPD amino acid sequence displayed conservation with homologues from other mesophilic eukaryotes with notable exceptions. In particular, an ice worm-specific K188E substitution proximal to the AMP binding site likely alters the architecture of the active site and negatively affects the enzyme's activity. Paradoxically, this would contribute to elevated intracellular ATP levels, which appears to be a signature of cold adapted taxa.

SUBMITTER: Marotta R

PROVIDER: S-EPMC3042610 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature

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Publications

Divergence of AMP Deaminase in the Ice Worm Mesenchytraeus solifugus (Annelida, Clitellata, Enchytraeidae).

Marotta Roberto R Parry Bradley R BR Shain Daniel H DH

International journal of evolutionary biology 20090705

Glacier ice worms, Mesenchytraeus solifugus and related species, are the largest glacially obligate metazoans. As one component of cold temperature adaptation, ice worms maintain atypically high energy levels in an apparent mechanism to offset cold temperature-induced lethargy and death. To explore this observation at a mechanistic level, we considered the putative contribution of 5' adenosine monophosphate deaminase (AMPD), a key regulator of energy metabolism in eukaryotes. We cloned cDNAs enc ...[more]

PMID: 21350654

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Project description:BackgroundSince the late 1980s, long-term monitoring of terrestrial ecosystems in metal-contaminated areas has been carried out in the Central Urals. As a part of these monitoring programmes, the data on soil macroinvertebrates in undisturbed areas as reference sites continues to be gathered. These data help study the local biodiversity and long-term dynamics of soil macroinvertebrate abundance in non-polluted areas.New informationThe dataset (available from the GBIF network at https://www.gbif.org/dataset/bf5bc7f6-71a3-4abd-8abc-861ee3cbf84a) includes information from a long-term monitoring programme for two taxa of Annelids, Lumbricidae and Enchytraeidae, which dwell in the topsoil of spruce-fir, birch, pine and floodplain forests in the Central Urals. The dataset includes information on the earthworm community structure (list of species, species abundance, number of egg cocoons, cocoon exuvia, juveniles and adults) and enchytraeid abundance. The dataset consists of 553 sampling events (= samples, corresponding to upper and lower layers of the soil monoliths) and 12739 occurrences (earthworms, mainly identified to species and earthworm cocoons and enchytraeids, identified to family) collected during 1990-1991, 2004, 2014-2016 and 2018-2020. In total, 3305 individuals of earthworms were collected, representing ten (out of twelve) species and all eight genera recorded for the fauna of the Central Urals. In addition, 7292 earthworm egg cocoons and cocoon exuvia and 6926 individuals of enchytraeids were accumulated. The presence-absence data on each of the ten earthworm species, egg cocoons, cocoon exuvia and enchytraeids are provided for each sampling event. All data were collected in undisturbed non-polluted areas and are used as a local reference for ecotoxicological monitoring. The dataset provides valuable information for estimating the composition and abundance of earthworm communities in different habitats over a long time and contributes to the study of soil fauna biodiversity in the Urals.

Dataset Information

Divergence of AMP Deaminase in the Ice Worm Mesenchytraeus solifugus (Annelida, Clitellata, Enchytraeidae).

Publications

Divergence of AMP Deaminase in the Ice Worm Mesenchytraeus solifugus (Annelida, Clitellata, Enchytraeidae).

Similar Datasets

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